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| 水蒸汽蒸馏法及顶空-固相微萃取法提取青蒿花挥发性成分比较分析 | |
| 引用本文： | 余正文,王伯初,杨占南,祝连彩. 水蒸汽蒸馏法及顶空-固相微萃取法提取青蒿花挥发性成分比较分析[J]. 光谱实验室, 2011, 28(3): 1276-1279 |
| 作者姓名： | 余正文 王伯初 杨占南 祝连彩 |
| 作者单位： | 1. 重庆大学生物工程学院,重庆市沙坪坝区沙正街174号,400030;贵州师范大学生命科学学院,贵阳市宝山北路116号,550001 2. 重庆大学生物工程学院,重庆市沙坪坝区沙正街174号,400030 3. 贵州师范大学贵州省山地信息系统与生态环境保护重点实验室,贵阳市宝山北路116号,550001 |
| 基金项目： | 重庆大学研究生创新基金 |
| 摘 要： | 采用水蒸汽蒸馏法(HD)及顶空固相微萃取法(HS-SPME)提取青蒿花的挥发性成分,GC-MS法分析其化学组成.已鉴定化合物占挥发性成分的95.01%(HD)和89.55%(HS-SPME).其中1.8-桉叶素(21.32%,20.96%),樟脑(24.34%,19.18%),莰烯(3.04%,5.26%),侧柏烯(3... |
| 关 键 词： | 青蒿花 顶空固相微萃取法 水蒸汽蒸馏法 气相色谱-质谱法 挥发性成分 |
Comparison for Extraction of the Volatile Composition in Artemisia Annua Flowers by Hydro-Distillation and Headspace Solid-Phase Microextraction Methods |
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| YU Zheng-Wena,b WANG Bo-Chua YANG Zhan-Nanc ZHU Lian-Cai. Comparison for Extraction of the Volatile Composition in Artemisia Annua Flowers by Hydro-Distillation and Headspace Solid-Phase Microextraction Methods[J]. Chinese Journal of Spectroscopy Laboratory, 2011, 28(3): 1276-1279 | |
| Authors: | YU Zheng-Wena b WANG Bo-Chua YANG Zhan-Nanc ZHU Lian-Cai |
| Affiliation: | aa(College of Bioengineering,Chongqing University,Chongqing 400030,P.R.China)b(School of Life Science,Guizhou Normal University,Guiyang 550001,P.R.China)c(Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou Province,Guizhou Normal University,Guiyang 550001,P.R.China) |
| Abstract: | The chemical components of essential oils that were extracted from Artemisia annua flowers by different methods were compared.The volatile extracts were obtained by traditional hydro-distillation（HD） and headspace solid-phase microextraction（HS-SPME） methods from Artemisia annua flowers,and the chemical components were analyzed by gas chromatograph-mass spectrometry（GC-MS） system.The identified components were 95.01%（HD） and 89.55%（HS-SPME） of the volatile chemical composition of flowers,respectively.The main constituents were 1,8-cineole（21.32%,20.96%）,camphor（24.34%,19.18%）,camphene（3.04%,5.26%）,sabinene（3.72%,4.08%）,α-terpineol（2.30%,1.16%）,β-farnesene（4.08%,1.62%） and 2-isopropenyl-4a,8-dimethyl-1,2,3,4,4a,5,6,7-octahydronaphthalene（3.40%,4.69%）,which were 62.20% and 56.95% of all volatile composition by HD and HS-SPME,respectively.There was no significant difference of the main chemical components,and their contents of the volatile composition were extracted by HD and HS-SPME.The HS-SPME method offers important advantages over traditional alternatives,namely：less samples,shorter isolation time,lower energy consumption,cleaner features.Furthermore,the extractions of volatile composition from HS-SPME could be analysed directly without any clean-up,solvent exchange or centrifugation steps.HS-SPME is a good method for the extraction of volatile composition from Artemisia annua flowers. |
| Keywords: | Artemisia Annua Flowers HS-SPME HD Gas Chromatography-Mass Spectrometry Volatile Composition |
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