Detection of small RNA molecules by a combination of branched rolling circle amplification and bioluminescent pyrophosphate assay |
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Authors: | Yasumasa Mashimo Masayasu Mie Shigeya Suzuki Eiry Kobatake |
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Institution: | (1) Grain Research Laboratory, Canadian Grain Commission, Winnipeg, MB, R3C 3G8, Canada;(2) Food Research Division, Health Canada, Banting Research Centre 2203D, 251 Sir Frederick Banting Driveway, Ottawa, ON, K1A 0L2, Canada; |
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Abstract: | Polyfluorinated compounds (PFCs) are a relatively new and diverse set of compounds analyzed as contaminants in food. Their
unique physical-chemical properties dictate the methods used for their analysis. Current analyses of the more volatile PFCs
involve gas chromatography–mass spectrometry; liquid chromatography–tandem mass spectrometry is generally used for the less
volatile PFCs. Considerations in the analysis of PFCs in foods include contamination from the widespread presence of materials
that contain various PFCs, endogenous interfering compounds, and matrix effects. Future opportunities for research on PFCs
in food exist, particularly in the areas of biological molecule–PFC interactions and the effects of food processing on these
interactions. Future research will be facilitated by the synthesis of a wider variety of analytical standards. |
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