Determination of polyamines in biological materials by HPLC with 9-fluorenylmethyl chloroformate precolumn derivatization |
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| Authors: | Gernot Huhn Jürgen Mattusch and Horst Schulz |
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| Institution: | (1) Department of Chemical Ecotoxicology, Centre for Environmental Research Leipzig-Halle, P.O. Box 2, D-04301 Leipzig, Germany;(2) Department of Analytical Chemistry, Centre for Environmental Research Leipzig-Halle, P.O. Box 2, D-04301 Leipzig, Germany |
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| Abstract: | Di- and polyamines have been determined in the femtomol range by liquid chromatography on a reversed-phase C8 column with a linear gradient of acetate buffer/acetonitrile as mobile phase and fluorimetric detection at 265/310 nm after simple derivatization with 9-fluorenylmethyl chloroformate (FMOC) within 3 min at pH 7.8 in presence of aspartic acid to remove excess reagent. The FMOC—polyamine-derivatives have been stable in a methanol—water-solution for at least 10 h. The method can be directly applied to crude plant extracts, and it is not interfered by carbohydrates and phenolics. |
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