| 循环肿瘤细胞捕获方法的研究进展 |
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| 引用本文： | 刘路宽,杨开广,梁振,张丽华,张玉奎. 循环肿瘤细胞捕获方法的研究进展[J]. 色谱, 2019, 37(4): 358-366. DOI: 10.3724/SP.J.1123.2018.12016 |
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| 作者姓名： | 刘路宽 杨开广 梁振 张丽华 张玉奎 |
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| 作者单位： | 1. 中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 辽宁 大连 116023;2. 中国科学院大学, 北京 100049;3. 大连理工大学, 精细化工国家重点实验室, 辽宁 大连 116024 |
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| 基金项目： | 国家重点研发计划（2017YFA0505003，2016YFA0501401）；国家自然科学基金（21575143，91543201，21725506）；中国科学院前沿科学重点研究项目（QYZDY-SSW-SLH017）；中国科学院青-创新促进会（2017222）. |
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| 摘 要： | 血液中的循环肿瘤细胞(CTCs)携带着肿瘤组织的遗传和表型信息,是液体活检的重要标志物。监测和分析血液中CTCs的数量和性质对癌症的早期诊断、治疗方案的确定和疗效评估具有重要意义。然而CTCs在血液中的含量极低,实现对CTCs的捕获与检测极具挑战。该文综述了基于生物物理原理、生物亲和原理以及人工抗体的CTCs捕获方法,并从捕获效率、捕获纯度和释放活性保持等方面进行了评述。此外,该文还对CTCs捕获方法的发展趋势进行了展望。
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| 关 键 词： | 循环肿瘤细胞 选择性捕获 生物物理 亲和 人工抗体 综述 |
| 收稿时间： | 2018-12-10 |
Advances in capture methods of circulating tumor cells |
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| LIU Lukuan,YANG Kaiguang,LIANG Zhen,ZHANG Lihua,ZHANG Yukui. Advances in capture methods of circulating tumor cells[J]. Chinese journal of chromatography, 2019, 37(4): 358-366. DOI: 10.3724/SP.J.1123.2018.12016 |
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| Authors: | LIU Lukuan YANG Kaiguang LIANG Zhen ZHANG Lihua ZHANG Yukui |
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| Affiliation: | 1. CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China |
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| Abstract: | Since circulating tumor cells (CTCs) in the blood carry the genetic and phenotypic information of tumor tissues, they are regarded as important markers for liquid biopsy, especially for the early diagnosis of cancer, determination of treatment options and evaluation of prognosis. However, the capture and detection of CTCs is challenging because of their extremely low concentration in blood. In this review, recent advances in capture methods of CTCs based on their biophysical properties, affinity, and artificial antibodies are summarized. The advantages and drawbacks of these methods are discussed from the aspects of capture efficiency, capture purity and maintenance of activity after release. Future trends in CTCs capture are also discussed. |
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| Keywords: | circulating tumor cells (CTCs) selective capture biophysics affinity artificial antibody review |
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