| 纳豆激酶分离纯化和酶活性测定的研究进展 |
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| 引用本文： | 法芸,张金玲,赵海杰,刘会洲.纳豆激酶分离纯化和酶活性测定的研究进展[J].色谱,2019,37(3):274-278. |
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| 作者姓名： | 法芸 张金玲 赵海杰 刘会洲 |
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| 作者单位： | 1. 中国科学院大学, 北京 100049;
2. 中国科学院青岛生物能源与过程研究所, 山东 青岛 266101;
3. 潍坊海关, 山东 潍坊 261041 |
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| 基金项目： | 国家自然科学基金（21405166）. |
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| 摘 要： | 该文对纳豆激酶的分离纯化和酶活性测定进行了综述。重点讨论了溶剂沉淀法、柱层析法、磁性微球吸附法、膨胀床法、反相胶束法、三相分割法等分离方法。对酶活性的不同测定方法进行了讨论和比较。提出了将核酸适配体识别技术用于纳豆激酶分离纯化和酶活性测定的可行性。
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| 关 键 词： | 分离纯化 纳豆激酶 酶活性 综述 |
| 收稿时间： | 2018-11-08 |
Progress in separation and purification of nattokinase and its enzyme activity determination |
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| FA Yun,ZHANG Jinling,ZHAO Haijie,LIU Huizhou.Progress in separation and purification of nattokinase and its enzyme activity determination[J].Chinese Journal of Chromatography,2019,37(3):274-278. |
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| Authors: | FA Yun ZHANG Jinling ZHAO Haijie LIU Huizhou |
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| Institution: | 1. University of Chinese Academy of Sciences, Beijing 100049, China;
2. Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China;
3. Weifang Customs, Weifang 261041, China |
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| Abstract: | In this paper, the separation and purification of nattokinase have been reviewed by primarily focusing on solvent precipitation, adsorption column chromatography, magnetic microspheres, expanded bed, reverse micelle, and three-phase separation methods. The different methods for determination of the enzyme activity have been discussed and compared. Finally, the feasibility that the nucleic acid-based identification techniques can be used for nattokinase purification and enzyme activity assay has been proposed. |
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| Keywords: | separation purification nattokinase enzyme activity review |
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