Nondenaturing polyacrylamide gel electrophoresis to study the dissociation of the p53·MDM2/X complex by potentially anticancer compounds |
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| Authors: | Roberta Sgammato Doriana Desiderio Anna Lamberti Gennaro Raimo Ettore Novellino Alfonso Carotenuto Mariorosario Masullo |
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| Institution: | 1. Department of Movement Sciences and Wellness, University of Naples “Parthenope”, Naples, Italy;2. CEINGE ‐ Biotecnologie Avanzate s.c. a r.l, Naples, Italy;3. Department of Biosciences and Territory, University of Molise, Pesche, Isernia, Italy;4. Department of Pharmacy, University of Naples Federico II, Naples, Italy |
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| Abstract: | A new analytical method to study the dissociation of the complexes between the oncosuppressor p53 and its negative modulators murine double‐minute protein 2 (MDM2) or MDMX, is proposed. This technique is reliable to determine the dissociative power exerted by small molecules on the complex taking advantage of the appearance of migrating MDM2 or MDMX in a native polyacrylamide gel, when inhibitors are added to the complex mixture. Therefore, we propose this new approach to easily screen library of compounds, with potential pharmacological anticancer activity. |
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| Keywords: | Complex dissociation Inhibitors MDM2 MDMX p53 |
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