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Intercalation of Proflavine in ssDNA Aptamers: Effect on Binding of the Specific Target Chloramphenicol |
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| Authors: | Magdalena Gebala Wolfgang Schuhmann Karolien De?Wael |
| Institution: | 1. Stanford School of Medicine, Department of Biochemistry, Beckmann Center Stanford, B465, 270 W. Campus Dr. MC:5307, Stanford, CA 94305, USA;2. Analytical Chemistry – Center for Electrochemical Sciences (CES), Ruhr‐Universit?t Bochum, Universit?tstr. 150, 44780 Bochum, Germany;3. University of Antwerp, AXES research group, Department of Chemistry, Groenenborgerlaan 171, 2020 Antwerp, Belgium |
| Abstract: | The structural modification of ssDNA‐based aptamers upon specific binding of its target molecule leads to changes of the charge‐transfer resistance (Rct) of a negatively‐charged free‐diffusing redox probe. The aptamer adopts a structure due to self‐hybridization which is stabilized using profalvine as intercalator. The pre‐organized aptamer structure is used to detect chloramphenicol (CAP) requiring a substantial change of the aptamer structure indicated by a CAP concentration dependent increase in the Rct values. Pre‐incubation of the aptamer‐modified electrode with an intercalator allows for the modulation of the aptamer/target interaction and hence for a modulation of the CAP‐dependent variation of the Rct values. |
| Keywords: | Aptamer Intercalator Proflavine Electrochemical impedance spectroscopy Chloramphenicol |