Electrochemical Activity of Wedelolactone and Probing its Interaction with DNA Using Voltammetry at a Carbon Electrode |
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| Authors: | Jiří Červeň Luděk Havran Petr Pečinka Miroslav Fojta |
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| Affiliation: | 1. University of Ostrava, Faculty of Science, Institute of Environmental Technologies, Department of Biology and Ecology, Chittussiho 10, CZ‐70103 Ostrava, Czech Republic;2. Institute of Biophysics, Academy of Sciences of the Czech Republic v.v.i. Kralovopolska 135, CZ‐61265 Brno, Czech Republic;3. CEITEC‐Central European Institute of Technology, Masaryk University, Kamenice 5, CZ‐62500, Brno, Czech Republic |
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| Abstract: | Wedelolactone (WLA) is a polyphenolic coumestan derivative found in extracts of plants used in traditional medicine. Due to its cytostatic activity, WLA is one of natural compounds tested as potential anticancer drugs. In this work we for the first time studied electrochemical properties of WLA using cyclic (CV) and square‐wave (SWV) voltammetry at the basal‐plane pyrolytic graphite electrode. A reversible pair of peaks, corresponding to catechol/o‐benzoquinone redox system, was observed using CV around 0.275 V vs. Ag|AgCl|3 M KCl reference electrode. Measurements of SWV signal of WLA in the presence of single‐ or double‐stranded DNA suggested a weak interaction without evident preference for double‐stranded DNA. An indirect assay, employing electroactive DNA intercalator doxorubicin as competitor, confirmed absence of intercalative DNA binding of WLA. |
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| Keywords: | Wedelolactone Electrochemistry Graphite electrode DNA Doxorubicin |
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