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Determination of Total PSA Using Magnetic Beads and a Re‐usable Screen Printed Carbon Electrode Array
Authors:Julien Biscay  María Begoña González García  Agustín Costa García
Institution:Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, 33006 Oviedo, Spain
Abstract:A total PSA (tPSA) assay using magnetic beads (1 µm) and a re‐usable 8‐channel screen printed electrochemical array is here reported. The reaction scheme is based on a one step sandwich assay. The linear range obtained is comprised between 5 and 100 ng/mL of tPSA and have a limit of detection of 1.86 ng/mL. This methodology was validated, performing determination of tPSA in serum and the results were compared with a commercial ELISA kit.
Keywords:magnetic beads  magnetic support  screen printed carbon electrode array  total PSA determination
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