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Counting of Escherichia coli by a microflow cytometer based on a photonic–microfluidic integrated device |
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| Authors: | Tianyi Guo Yin Wei Changqing Xu Benjamin R Watts Zhiyi Zhang Qiyin Fang Haiying Zhang P Ravi Selvaganapathy M Jamal Deen |
| Institution: | 1. School of Biomedical Engineering, McMaster University, Hamilton, Canada;2. Institute of Microelectronics, Chinese Academy of Science, Beijing, China;3. Department of Engineering Physics, McMaster University, Hamilton, Canada;4. Information and Communication Technologies, National Research Council of Canada, Ottawa, Canada;5. Department of Mechanical Engineering, McMaster University, Hamilton, Canada;6. Department of Electrical and Computer Engineering, McMaster University, Hamilton, Canada |
| Abstract: | Counting of Escherichia coli DH5α‐cell suspensions in PBS is performed using a microflow cytometer based on a photonic–microfluidic integrated device. Side‐scattered light signals are used to count the E. coli cells. A detection efficiency of 92% is achieved when compared with the expected count from a hemocytometer. The detection efficiency is correlated to the ratio of sample to sheath flow rates. It is demonstrated that E. coli can be easily distinguished from beads of similar sizes (2–4 μm) as their scattering intensities are different. |
| Keywords: | Escherichia coli Integrated optics Microflow cytometer Scattering detection |