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Construction and Application of Flow Enzymatic Biosensor Based of Silver Solid Amalgam Electrode for Determination of Sarcosine |
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| Authors: | Oksana Josypčuk Jiří Barek Bohdan Josypčuk |
| Affiliation: | 1. Charles University in Prague, Faculty of Science, University Research Center UNCE “Supramolecular chemistry”, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, CZ‐128 43 Prague 2, Czech Republic;2. J. Heyrovsky Institute of Physical Chemistry of AS CR, v.v.i., Department of Biomimetic Electrochemistry, Dolejskova 3, Prague, Czech Republic |
| Abstract: | In this paper, the flow amperometric enzymatic biosensor based on polished silver solid amalgam electrode for determination of sarcosine in model sample under flow injection analysis conditions is presented. The biosensor works on principle of electrochemical detection of oxygen decrease during enzymatic reaction which is directly proportional to the concentration of sarcosine in sample. The whole preparation process takes about 3 h. The RSD of repeatability of 10 consecutive measurements is 1.6 % (csarcosine=1.0×10?4 mol dm?3). Under optimal conditions the calibration dependence was linear in the range 7.5×10?6–5.0×10?4 mol dm?3 and limit of detection was 2.0×10?6 mol dm?3. |
| Keywords: | Biosensors Sarcosine Silver solid amalgam electrode Electrochemistry Amperometry |