Amperometric Ethanol Biosensors Based on Chitosan‐NAD+‐Alcohol Dehydrogenase Films |
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Authors: | Maogen Zhang Waldemar Gorski |
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Institution: | Department of Chemistry, University of Texas at San Antonio, San Antonio, Texas 78249‐0698, USA |
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Abstract: | The reagentless and oxygen‐independent biosensors for ethanol were developed based on the covalent immobilization of alcohol dehydrogenase (ADH) and its cofactor nicotinamide adenine dinucleotide (NAD+) on chitosan (CHIT) chains. The CHIT‐NAD+‐ADH structures were adsorbed onto carbon nanotubes (CNT) in order to provide a signal transduction based on the recycling of redox states of NAD cofactor at CNT (detection limit, 8–30 µM ethanol; dynamic range up to 20 mM). The CHIT‐NAD+‐dehydrogenase/CNT hybrid material represents a general approach to the development of dehydrogenases‐based electrochemical biosensors. Interestingly, the CHIT‐NAD+ solutions preserved their enzymatic activity even after five years of storage at 4 °C. |
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Keywords: | Carbon nanotubes Enzymatic activity Ethanol Biosensor Dehydrogenase |
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