A Polymer‐Based Electrochemical DNA Biosensor for Salmonella: Preparation,Characterization and Calibration |
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| Authors: | Madeline Díaz‐Serrano Arelys Rosado Joselyn del Pilar Melvin Arias Ana R Guadalupe |
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| Institution: | 1. Department of Chemistry, P.?O. Box 23346, University of Puerto Rico, Río Piedras Campus, San Juan, Puerto Rico, 00931‐3346;2. Department of Physics and Mathematics, Antonio R. Barceló Ave., University of Puerto Rico, Cayey Campus, Cayey, Puerto Rico, 00736, Presently at Santo Domingo Institute of Technology (INTEC), 342‐9, Santo Domingo, Dominican Republic |
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| Abstract: | In this paper, we present the results of the use of bifunctional polymeric films of polystyrene (10.3 KD–49.5 KD) to anchor oligo sequences of various lengths (15, 35 and 70‐mer). The polymers were prepared by radical polymerization with 4,4′‐azobis(4‐cyanovaleric acid) as initiator and 3‐Carboxy PROXYL to control the molecular weight and polydispersity. They were further modified with N‐hydroxysuccinimide to anchor the (5′‐AmMC12) oligos. The anchoring reaction was done on a polymer‐modified glassy carbon electrode. The probes were hybridized with their ferrocene‐labeled complementary sequences. The hybridization reaction was followed by Osteryoung square wave voltammetry (OSWV). The calibration curve showed a narrow and sharp linear range between (5.7–8.0)×10?7 M and a detection limit around 0.55 µM. |
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| Keywords: | AFM Biosensors Electrochemical detection Nucleic acids Polymer modified electrodes Salmonella |
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