Synthesis of Thioproline Salicylic Acid Samarium Complex and Microcalorimetric Study on Effects of the Complex on the Growth Metabolism of S. pombe Cells

The product from reaction of samarium chloride hexahydrate with salicylic acid and thioproline, Sm(C₇H₅O₃)₂·(C₄H₆NO₂S)]·2H₂O (TSAS), was synthesized and characterized by IR, elemental analysis and thermogravimatric analysis. Particularly, the effect of the compounds TSAS, SmCl₃·6H₂O, C₇H₆O₃ and C₄H₇NO₂S on the growth and metabolism of S. pombe by a TAM Air isothermal calorimeter at 32°C is also reported. The thermokinetic parameters, which included the microbial growth rate constant (κ), inhibition ratio (I) and half inhibition concentration (IC₅₀), were obtained. The results showed that all the compounds TSAS, SmCl₃·6H₂O, C₇H₆O₃ and C₄H₇NO₂S possessed the bi‐directional biological effect and Hormesis effect. These stimulate the growth of the S. pombe at lower concentration, but inhibit the growth at higher concentration. The half inhibition concentrations (IC₅₀) of TSAS, SmCl₃·6H₂O, C₄H₇NO₂S and C₇H₆O₃ were found to be 0.732, 0.746, 0.746 and 1.43 mmol·L⁻¹, respectively. The inhibition ability of these compounds on the growth of the S. pombe has been observed to decrease in the order TSAS>SmCl₃·6H₂O=C₄H₇NO₂S>C₇H₆O₃. Moreover, the effect of TSAS on the growth and cytokinesis of S. pombe was investigated by cytomorphology and FT IR spectroscopy technique. The results indicated that TSAS had a direct impact on apoptosis, and could also inhibit the cell growth by reducing cytokinesis and the binding locations of drug (TSAS) in S. pombe cells could be the proteins and nucleic acid.