Electrochemical Behavior and Electroanalytical Determination of Indole‐3‐Acetic Acid Phytohormone on a Boron‐Doped Diamond Electrode |
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| Authors: | Yavuz Yardım M. Emre Erez |
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| Affiliation: | 1. Yüzüncü Y?l University, Faculty of Science and Art, Department of Analytical Chemistry, 65080 Van, Turkey tel: +90?432?2251024;2. fax: +90?432?2251188;3. Yüzüncü Y?l University, Faculty of Science and Art, Department of General Biology, 65080 Van, Turkey |
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| Abstract: | In this work, a boron‐doped diamond (BDD) electrode was used for the electroanalytical determination of indole‐3‐acetic acid (IAA) phytohormone by square‐wave voltammetry. IAA yielded a well‐defined voltammetric response at +0.93 V (vs. Ag/AgCl) in Britton–Robinson buffer, pH 2.0. The process could be used to determine IAA in the concentration range of 5.0 to 50.0 µM (n=8, r=0.997), with a detection limit of 1.22 µM. The relative standard deviation of ten measurements was 2.09 % for 20.0 µM IAA. As an example, the practical applicability of BDD electrode was tested with the measurement of IAA in some plant seeds. |
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| Keywords: | Indole‐3‐acetic acid Boron‐doped diamond electrode Square‐wave voltammetry Plant hormones |
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