An enzyme electrode for the amperometric determination of glucose

An amperometric method utilizing a glucose electrode has been developed for the determination of blood glucose. The time of measurement is less than 12 s if a kinetic method is used and 1 min if a steady-state method is used. The long-term stability of the electrode is ca. 0.1% change from maximum response per day when stored at room temperature for over 10 months. The enzyme electrode determination of blood sugar compares favorably with commonly used methods with respect to accuracy, precision, and stability. The only reagent required for blood sugar determinations is a buffer solution. The electrode consists of a metallic sensing layer covered by a thin film of immobilized glucose oxidase held in place by means of cellophane. When poised at the correct potential, the current produced is proportional to the glucose concentration.