| 乳制品中那他霉素的超高效液相色谱-串联质谱法快速测定 |
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| 引用本文： | 周莉莉,祝建华,张卉,王骏,于文江,范晓明. 乳制品中那他霉素的超高效液相色谱-串联质谱法快速测定[J]. 分析测试学报, 2010, 29(1). DOI: 10.3969/j.issn.1004-4957.2010.01.018 |
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| 作者姓名： | 周莉莉 祝建华 张卉 王骏 于文江 范晓明 |
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| 作者单位： | 山东省产品质量监督检验研究院,国家加工食品质量监督检验中心(山东),山东,济南,250100;山东省产品质量监督检验研究院,国家加工食品质量监督检验中心(山东),山东,济南,250100;山东省产品质量监督检验研究院,国家加工食品质量监督检验中心(山东),山东,济南,250100;山东省产品质量监督检验研究院,国家加工食品质量监督检验中心(山东),山东,济南,250100;山东省产品质量监督检验研究院,国家加工食品质量监督检验中心(山东),山东,济南,250100;山东省产品质量监督检验研究院,国家加工食品质量监督检验中心(山东),山东,济南,250100 |
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| 摘 要： | 建立了超高效液相色谱-电喷雾串联质谱(UPLC-MS/MS)快速检测乳制品中那他霉素的方法。样品用甲醇提取,以甲醇-水为流动相经反相色谱柱分离后,采用多反应监测(MRM)负离子模式检测,定性离子对为m/z663.6/421.1和m/z663.6/439.1,其中m/z663.6/421.1用于外标法定量。空白样品及其加标实验结果表明:特征离子相对强度比值稳定,无基质干扰,结合保留时间可实现准确的定性定量;方法定量下限为50.0μg/kg;乳制品加标量为50~500μg/kg时,平均回收率为80%~91%,相对标准偏差(n=6)为2.7%~5.2%。方法简单、灵敏、稳定,可满足乳制品中那他霉素的快速检测与确证需要。
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| 关 键 词： | 那他霉素 超高效液相色谱-串联质谱 乳制品 |
Rapid Determination of Natamycin in Milk Products by Ultra-performance Liquid Chromatography-Electrospray Negative Ionization Mass Spectrometry |
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| ZHOU Li-li,ZHU Jian-hua,ZHANG Hui,WANG Jun,YU Wen-jiang,FAN Xiao-ming. Rapid Determination of Natamycin in Milk Products by Ultra-performance Liquid Chromatography-Electrospray Negative Ionization Mass Spectrometry[J]. Journal of Instrumental Analysis, 2010, 29(1). DOI: 10.3969/j.issn.1004-4957.2010.01.018 |
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| Authors: | ZHOU Li-li ZHU Jian-hua ZHANG Hui WANG Jun YU Wen-jiang FAN Xiao-ming |
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| Affiliation: | ZHOU Li-li,ZHU Jian-hua,ZHANG Hui,WANG Jun,YU Wen-jiang,FAN Xiao-ming(National Center for Quality Supervision , Inspection of Processed Food(Sh,ong),Sh,ong Supervision , Inspection Institute for Product Quality,Jinan 250100,China) |
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| Abstract: | A rapid ultra-performance liquid chromatography-tandem mass spectrometric(UPLC-MS/MS) method was developed for the determination of natamycin in milk products.The sample was extracted by methanol,and separated on a reversed-phase UPLC column using a binary eluent under gradient conditions.The analysis of natamycin was performed by tandem mass spectrometry under multiple reaction monitoring(MRM) mode with negative electrospray ionization.Two precursor-product ion pairs of m/z 663.6/421.1 and m/z 663.6/439.1 ... |
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| Keywords: | natamycin UPLC-MS/MS milk products |
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