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Facile Chemoenzymatic Synthesis of O‐Mannosyl Glycans |
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| Authors: | CongCong Chen Yuxi Guo Dr Madhusudhan Reddy Gadi Dr Jin Yu Prof?Dr Ulrika Westerlind Dr Yunpeng Liu Dr Xuefeng Cao Prof?Dr Peng G Wang Dr Lei Li |
| Institution: | 1. National Glycoengineering Research Center, School of Pharmaceutical Science, Shandong University, Jinan, China;2. Department of Chemistry & Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA, USA;3. Leibniz-Institut für Analytische Wissenschaften—ISAS—e.V., Dortmund, Germany;4. Department of Chemistry, Ume? University, Ume?, Sweden |
| Abstract: | O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O‐mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms. An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram‐scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases. In total, 45 O‐mannosyl glycans were obtained, covering the majority of identified mammalian structures. Subsequent glycan microarray analysis revealed fine specificities of glycan‐binding proteins and specific antisera. |
| Keywords: | carbohydrates chemoenzymatic synthesis glycosylation microarrays O-mannosyl glycans |