Effects of interactions on the formation of mixed micelles of 1,2-diheptanoyl-sn-glycero-3-phosphocholine with sodium dodecyl sulfate and dodecyltrimethylammonium bromide

Mixed micelles of the phospholipid 1,2-diheptanoyl-sn-glycero-3-phosphocholine (DHPC) with sodium dodecyl sulfate (SDS) or dodecyltrimethylammonium bromide (DTAB) in aqueous solutions and the effects of interactions between the components were studied by fluorescence and NMR measurements. The regular solution theory (RST) was applied to analyze the experimental critical micelle concentration values determined from the fluorescence spectra of pyrene in the mixed micelles. Negative values for the interaction parameter (beta12) were obtained for both DHPC + SDS and DHPC + DTAB mixtures, with the value being more negative in the former case. The negative beta12 values for the two systems imply that the interaction between the phospholipid and the two ionic surfactants is attractive in nature, being more intense in the case of DHPC + SDS. The interaction parameter, beta12, varies with composition of the mixtures indicating changes in packing. The proton NMR shifts are quite different for the two systems and also vary with composition. An interpretation of these experimentally determined chemical shifts in terms of the degree of compactness attributed to electrostatic and steric interactions in the mixed micelle supports the conclusions derived from the fluorescence cmc experiments.