| 毛细管电泳接触反应法测定血清中铁传递蛋白的浓度 |
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| 引用本文： | 丁永生.毛细管电泳接触反应法测定血清中铁传递蛋白的浓度[J].分析化学,1998,26(5):524-527. |
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| 作者姓名： | 丁永生 |
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| 作者单位： | 中国科学院大连化学物理研究所!大连116023，中国科学院大连化学物理研究所!大连116023，DivisionofScience，TrumanStateUniversity，Kirksville!MO63501，USA |
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| 基金项目： | 国家自然科学基金，交通部教育司基金资助项目 |
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| 摘 要： | 建立了一种测定血清中铁传递蛋白的毛细管电泳接触反应方法，利用血清中铁传递蛋白计算化学结合铁的性质，用标准铁饮和铁传递蛋通过对铁准确定量，测定血清铁伟递蛋白的有浓度。
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| 关 键 词： | 铁传递蛋白 血清 毛细管电泳 接触反应法 分析 |
Catalytic Method Determination of Serum Transferrin by Capillary Electrophoresis |
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| Ding Yongsheng,Ma Yinfa,Lin Bingcheng.Catalytic Method Determination of Serum Transferrin by Capillary Electrophoresis[J].Chinese Journal of Analytical Chemistry,1998,26(5):524-527. |
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| Authors: | Ding Yongsheng Ma Yinfa Lin Bingcheng |
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| Abstract: | A high performance capillary electrophoresis (HPCE) catalytic method for the determination of serum transferrin concentration has been developed. The whole process includes techniques of pretreatment of serum samples and HPCE system optimization. For standard solution, a good linear response was maintained over the range 0. 5-10 umol/L (r = 0. 995). Seven healthy adult serum samples were studied by this system and their transferrin concentrations are 23. 9, 25. 7, 26. 9, 24. 5, 23. 2, 25. 5 and 23. 7 umol/L. 30 uL serum would be sufficient for transferrin measurement and the total serum pretreatment process takes less than 2 hours and the CE separation process takes only 6 min. This HPCE method is reliable, sensitive, rapid and efficient. Thus it is suitable for clinical application. |
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| Keywords: | Capillary electrophoresis serum transferrin iron catalytic method |
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