Determination of triazine herbicides in human body fluids by solid-phase microextraction and capillary gas chromatography |
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Authors: | T Kumazawa X -P Lee K Kondo K Sato H Seno K Watanabe-Suzuki A Ishii O Suzuki |
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Institution: | (1) Department of Legal Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, 142-8555 Tokyo, Japan;(2) Department of Legal Medicine, Hamamatsu University School of Medicine, 3600 Handa-cho, 431-3192 Hamamatsu, Japan;(3) Department of Legal Medicine and Bioethics, Nagoya University Post Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, 466-8550 Nagoya, Japan |
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Abstract: | Summary Eight triazine herbicides, prometon, propazine, atrazine, simazine, prometryn, ametryn, metribuzin, and cyanazine, have been
extracted from human whole blood and urine samples by headspace solid-phase microextraction (SPME) with a polydimethylsiloxane-coated
fiber and quantified by capillary gas chromatography with nitrogen-phosphorus detection.
Extraction efficiencies for all compounds were 0.21–0.99% for whole blood, except for cyanazine (0.06%). For urine, the extraction
efficiencies for prometon, propazine, atrazine, prometryn and ametryn were 13.6–38.1%, and those of simazine, metribuzin and
cyanazine were 1.35–8.73%.
The regression equations for the compounds extracted from whole blood were linear within the concentration ranged 0.01–1 μg
(0.5 mL)−1 for prometon, propazine, atrazine, prometryn, and ametryn, and 0.02–1 μg (0.5 mL)−1 for simazine, metribuzin, and cyanazine. For urine, regression equations for all compounds were linear within the concentration
range 0.005–0.25 μg mL−1. Compound detection limits were 2.8–9.0 ng (0.5 mL)−1 and 0.4–2.0 ng mL−1 for whole blood and urine, respectively. The coefficients of within-day and day-to-day variation were satisfactory for all
the compounds, and not greater than 10.3 and 14.2%, respectively.
Data obtained from determination of atrazine in rat whole blood after oral administration of the compound are also presented. |
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Keywords: | Gas chromatography Solid-phase microextraction Blood and urine samples Nitrogen-phosphorus (NP) detection Triazine herbicides |
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