聚乙烯醇载体的研究 Ⅲ.由聚乙烯醇衍生的活性酰胺和活性酯及其作为亲合色谱载体的应用

<正> 亲合色谱法在现代生物学和医学上作为新的得力分离分析手段而方兴未艾。合色谱法是将相互在生物学上具有特异亲合力的某一化合物作为固定相接在水不溶性载体上,利用对此固定相的亲合力的差异以分离提纯另一化合物为目的的方法。常用的水不溶性载体如天然纤维素,葡聚糖凝胶,琼脂糖凝胶等或因成型不易,或因对酸,霉菌等的不

STUDIES ON POLYYINYL ALCOHOL CARRIERS Ⅲ.Active Amides and Active Esters Derived from Poly-Vinyl Alcohol and Thier Uses as the Carriers for Affinity Chromatography

Polyvinyl alcohol carriers having active amide and active ester ligand were prepared by acetalization of granular macro-reticular water-insoluble PVA with glyoxylic acid or p-form ylbenzoic aeid, and then reaction with benzotriazole or N-hydroxysuccinimide by using DCCI as condensation agent. The active amides or active esters obtained are easy to react with amines such as n-butylamine or proteines under mild condition.Ovomucoid, an inhibitor of trypsin was immobilized on the above obtained reactive PVA carrier to give an effective affinity-adsorbent for separation of trypsin.