The estimation of leucine aminopeptidase produced by molting nematodes with labeled leucinamide as substrate

Leucine aminopeptidase activity was measured using l-U-¹⁴C]leucinamide as substrate. The leucine freed by hydrolysis was separated by high voltage electrophoresis and estimated in a scintillation counter. The method was especially useful for studying the production of LAP during the molting of infective juveniles of the nematodes, H. contortus, because activity could be measured without purifying or concentrating the enzyme.