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Laying the groundwork for proteomics: Mass spectrometry from 1958 to 1988
Institution:1. Division of Cardiology, Thomas Jefferson University Hospital, Philadelphia, Pennsylvania;2. Duke Clinical Research Institute, Durham, North Carolina;3. Merck Sharp & Dohme Corp., Whitehouse Station, New Jersey;4. Heart Institute, Hadassah–Hebrew University Medical Center, Jerusalem, Israel;6. Thoraxcentre, Rotterdam, the Netherlands;5. Department of Medical Sciences, Uppsala Clinical Research Center, Uppsala, Sweden;7. Department of Cardiovascular Science, University of Sheffield, Sheffield, United Kingdom;11. Department of Coronary Artery Disease and Cardiac Catheterization Laboratory, Institute of Cardiology, Warsaw, Poland;12. 3rd Department of Medicine, Cardiology and Emergency Medicine, Wilhelminen Hospital, Vienna, Austria;8. Department of Cardiology, University of Leuven, Leuven, Belgium;10. Institut Universitaire de Cardiologie et de Pneumologie de Québec, Québec City, Québec, Canada;9. Gill Heart Institute and Division of Cardiovascular Medicine, University of Kentucky, Lexington, Kentucky;71. Green Lane Cardiovascular Service, Auckland City Hospital, Auckland, New Zealand;112. Division of Cardiology, University of Alberta, Edmonton, Alberta, Canada;123. Department of Medicine, Stanford University, Stanford, California;1. University of Alicante, Department of Analytical Chemistry, Nutrition and Food Sciences, PO Box 99, 03080 Alicante, Spain;2. Ghent University, Department of Analytical Chemistry, Krijgslaan 281-S12, 9000 Ghent, Belgium;3. Delft University of Technology, Faculty of Applied Sciences, Analytical Biotechnology, Julianalaan 67, 2628 BC Delft, The Netherlands;1. School of Chemistry and Biochemistry, University of Western Australia, Crawley, Western Australia 6009, Australia;2. Centre for Microscopy, Characterisation and Analysis, University of Western Australia, Crawley, Western Australia 6009, Australia;1. Department of Industrial Pharmacy, Federal University of Santa Maria, 97105-900 Santa Maria-RS, Brazil;2. Postgraduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, 97105-900 Santa Maria-RS, Brazil;1. Department of Biophysics, Panjab University, Chandigarh 160014, India;2. Department of Physics, Panjab University, Chandigarh 160014, India;1. Studies Coordinating Centre, Research Unit Hypertension and Cardiovascular Epidemiology, KU Leuven Department of Cardiovascular Diseases, University of Leuven, Leuven, Belgium;2. Program of Cardiovascular Diseases, Centre for Applied Medical Research, University of Navarra, Navarra Institute for Health Research, Pamplona, Spain;3. CIBERCV, Carlos III Institute of Health, Madrid, Spain;4. Mosaiques Diagnostic and Therapeutics AG, Hannover, Germany;5. Research Unit Cardiology, KU Leuven Department of Cardiovascular Diseases, University of Leuven, Leuven, Belgium;6. Centre for Molecular and Vascular Biology, KU Leuven Department of Cardiovascular Diseases, University of Leuven, Leuven, Belgium;7. Department of Cardiology and Cardiac Surgery, University of Navarra Clinic, Pamplona, Spain;8. BHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow, United Kingdom;9. R&D Group VitaK, Maastricht University, Maastricht, The Netherlands
Abstract:The development of mass spectrometric methodologies for the sequencing of peptides and proteins are recounted. Early strategies for the determination of very large proteins based on a combination of nucleotide sequencing and mass spectrometric amino acid sequencing are described and their historical significance to the new field of proteomics is outlined.
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