Improved enzyme immobilization for enhanced bioelectrocatalytic activity of porous electrodes |
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| Institution: | 1. University Bordeaux 1, CNRS, ISM, Ecole Nationale Supérieur de Chimie et Physique de Bordeaux, 16 Avenue Pey Berland, 33607 Pessac, France;2. Department of Physical Chemistry, Universität des Saarlandes, 66123 Saarbrücken, Germany;3. Department of Material Science, Universität des Saarlandes, 66123 Saarbrücken, Germany;4. Centre de Recherche Paul Pascal-CRPP, 115 Avenue du Dr. Schweitzer, 33600 Pessac, France;5. Department of Analytical Chemistry, Ruhr-Universität Bochum, Universitätsstr. 150, 44780 Bochum, Germany;1. Laboratorio de Bioelectroquimica. Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile;2. Advanced Center for Chronic Diseases (ACCDiS). Universidad de Chile;3. Instituto de Carboquímica, CSIC, c/Miguel Luesma, 450018 Zaragoza, Spain;1. Departamento de Engenharia Mecânica, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, Rua Luís Reis Santos, 3030-788 Coimbra, Portugal;2. Faculty of Physics, University of Bucharest, 077125 Magurele-Bucharest, Romania;1. School of Advanced Materials Engineering and Research Center for Advanced Materials Development (RCAMD), Chonbuk National University, Chonju 561-756, Republic of Korea;2. Convergence Components & Materials Research Laboratory, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700, Republic of Korea;3. LED Device Research Center, Korea Photonics Technology Institute, Gwangju 500-779, Republic of Korea;1. Department of Statistics, Texas A&M University, USA;2. Department of Statistics, Korea University, Republic of Korea;3. Department of Statistics, Pukyong National University, Republic of Korea;1. College of Life Science and Technology, Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, Jinan University, Guangzhou 510532, China;2. Third Institute of Oceanography, SOA, Xiamen 361005, China;3. Zhongshan Entry-Exit Inspection and Quarantine Bureau Technology Center, Zhongshan 528403, China |
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| Abstract: | Porous electrodes with increased surface area have been prepared using a template route via colloidal crystals. The ordered porous structure and the interconnections between the pores have been quantitatively characterized by Focused Ion Beam Tomography. The internal surfaces of the electrodes have been biofunctionalized with two enzymatic systems for glucose oxidation. In order to increase significantly the stability, the biocatalysts have been immobilized either by crosslinking or by incorporation in an electrodeposition paint. The modified porous electrodes show an increased overall signal and therefore a better detection limit and a higher sensitivity when used as sensors, and a potentially higher power output when employed in biofuel cells. |
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