Direct electrochemistry and electrocatalysis of hemoglobin immobilized in bimodal mesoporous silica and chitosan inorganic–organic hybrid film |
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Affiliation: | 1. School of Chemistry & Chemical Engineering, Southeast University, 211189 Nanjing, China;2. Southeast University Affiliated Zhongda Hospital, Southeast University, Nanjing 210009, China;3. Départment de Chimie Moléculaire UMR-5250, ICMG FR-2607, CNRS Université Joseph Fourier, BP-53, 38041 Grenoble, France |
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Abstract: | Hemoglobin modified electrode was successfully fabricated to realize direct electrochemistry by immobilizing of Hemoglobin (Hb) in bimodal mesoporous silica (BMS) and chitosan (CS) inorganic–organic hybrid film. Here, BMS acted as a support to immobilize Hb due to its large pores and CS acted as a binder to increase film adherence and stabilizer to prevent the leakage of Hb. The resulting electrode (Hb/BMS/CS) gave a well-defined, reversible redox couple for HbFe(III)/Fe(II) with a formal potential of about −0.32 V (vs. Ag/AgCl) in pH 7.0 phosphate buffer solution. Hb/BMS/CS electrode showed a better electrocatalytial performance to H2O2 with wider linear detection range, lower detection limit, and higher sensitivity than that at electrode without BMS. The improved electrocatalytic performance for Hb/BMS/CS electrode was possibly contributed to BMS bimodal structure, whose large pores with 10–40 nm provide favorable conditions for protein immobilization and small pores with 2–3 nm avoid the mass-transfer limitations. In addition, UV–Vis and FTIR spectra indicated that Hb well maintained its native structure in the hybrid film. |
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