Construction of an amperometric biosensor for xanthine via supramolecular associations |
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| Affiliation: | 1. Department of Physical Chemistry, Palacky University, tr. Svobody 26, 77146 Olomouc, Czech Republic;2. Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 77515 Olomouc, Czech Republic;3. Department of Physical Chemistry, Institute of Chemical Technology, Prague, Technická 5, 16628 Prague 6, Czech Republic;4. Department of Inorganic and Organic Chemistry, Charles University, Faculty of Pharmacy, Heyrovskeho 1203, CZ-500 05 Hradec Kralove, Czech Republic;5. Institute of Microbiology, Department of Phototrophic Microorganisms, Academy of Sciences of the Czech Republic, Opatovický Mlýn, 37981 Třeboň, Czech Republic |
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| Abstract: | Xathine oxidase was chemically modified with β-cyclodextrin-branched carboxymethylcellulose and further supramolecularly immobilized on a gold electrode, previously coated with a monolayer of 1-adamantanyl residues. The electrode was employed for constructing an amperometric biosensor device, which showed linear response (poised at +700 mV vs. Ag/AgCl) toward xanthine concentration between 300 μM and 10.4 mM at pH 7.0. The biosensor reached 95% of steady-state current in about 14 s and its sensitivity was 8.2 mA/M cm2. The enzyme electrode retained 93% of its initial activity after 3 weeks of storage at 4 °C in 50 mM sodium phosphate buffer, pH 7.0. The supramolecular nature of the immobilization approach was confirmed by cyclic voltammetry. |
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