Assembly of electroactive layer-by-layer films of myoglobin and small-molecular phytic acid |
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| Institution: | 1. Department of Environmental Engineering, Chosun University, Gwangju 61452, Republic of Korea;2. Department of Civil and Environmental Engineering, Pusan National University, Busan 46241, Republic of Korea;3. Department of Civil and Environmental Engineering, University of New Haven, West Haven, CT 06516, USA;4. Korea Biochar Research Center, O-Jeong Eco-Resilience Institute (OJERI) & Division of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea;5. Institute of Mine Reclamation Technology, Mine Reclamation Corporation, Wonju 26464, Republic of Korea;6. Department of Environmental and Energy Engineering, Chonnam National University, Gwangju 61186, Republic of Korea;1. State Key Laboratory of Fire Science, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, People''s Republic of China;2. Suzhou Key Laboratory of Urban Public Safety, Suzhou Institute of University of Science and Technology of China, 166 Ren’ai Road, Suzhou, Jiangsu 215123, People''s Republic of China;3. Department of Building and Construction, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong;1. Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada;2. Instituto de Ganadería de Montaña (IGM) CSIC-Universidad de León, Departamento de Producción Animal, Universidad de León, E-24071, León, Spain;3. Department of Soil Science, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada;4. Centre for Nutrition Modelling, Department of Animal Biosciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada;5. Department of Animal Science, University of California, Davis, CA, 95616, USA |
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| Abstract: | In the present work, small-molecular phytic acid (PA) with its unique structure was successfully assembled with myoglobin (Mb) into {PA/Mb}n layer-by-layer films on solid surfaces. Quartz crystal microbalance (QCM) and cyclic voltammetry (CV) were used to monitor or confirm the assembly process. IR and UV–vis spectroscopy indicate that the Mb in {PA/Mb}n films retains its near native structure. The direct electrochemistry of Mb was realized in this new kind of films at pyrolytic graphite (PG) electrodes, and was used to electrocatalyze the reduction of various substrates. The interaction between PA and Mb under different pH conditions was also explored. Not only the oppositely charged PA and Mb at pH 5.0, but also the likely charged PA and Mb at pH 9.0, could be assembled into {PA/Mb}n films. This work provides a novel avenue to fabricate protein multilayer films with small molecules and realizes the direct electrochemistry of redox proteins in the films. |
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