Functionalization of multi-walled carbon nanotubes with poly(amidoamine) dendrimer for mediator-free glucose biosensor |
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| Institution: | 1. State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, 410082 Changsha, China;2. School of Chemistry and Chemical Engineering, Science and Technology of Hunan University, 411201 Xiangtan, China;1. Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500, Turkey;2. Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul 34500, Turkey;1. Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu-city 41566, Republic of Korea;2. School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, PR China;1. National Center of Excellence in Physical Chemistry, University of Peshawar, 25120 Peshawar, Pakistan;2. Institute of Chemical Sciences, University of Peshawar, 25120 Peshawar, Pakistan;1. Institute of Atomic Physics and Spectroscopy, University of Latvia, Jelgavas Street 3, Riga, LV-1004, Latvia;2. Center for Collective Use of Scientific Equipment, Sumy State University, 31, Sanatornaya st., 40018 Sumy, Ukraine;3. Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania |
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| Abstract: | By grafting with poly(amidoamine) (PAMAM) dendrimer, novel carbon nanotube (CNT) nano-composites have been successfully prepared. The novel functionalized matrix with plenty amino groups circumvents the troublesome solubility problem of CNTs in solvents, especially in water, greatly expanding the scope of the application of carbon nanotubes. The GOx and HRP immobilized CNT-PAMAM based on the functional CNTs was synthesized. The bi-enzymatic CNT-PAMAM nano-composites are highly dispersible in water and show very promising applications in the fabrication of mediator-free bi-enzymatic biosensors for sensitive detection of glucose. The cooperation of nano-composite between CNT and high dense GOx and HRP results in very high sensitivity to glucose with a current response of 2200 nA mM?1 and fast response (～1 s). The modified electrode exhibits a wide linear response range for glucose from 4.0 μM to 1.2 mM (R = 0.9971, N = 15), with a detection limit of 2.5 μM. The negative electrode potential of ?0.34 V is favorable for glucose detection in real samples without interference caused by other biomolecules. |
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