A new method for detection of endotoxin on polymyxin B-immobilized gold electrodes |
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| Institution: | 1. Institute of Oral Materials Science, Chung-Shan Medical University, Taichung 402, Taiwan, ROC;2. Department of Healthcare Administration, Hung-Kuang University, Taichung 433, Taiwan, ROC;3. Department of Bio-Industrial Mechatronics Engineering, National Chung-Hsing University, Taichung 402, Taiwan, ROC;4. Institute of Biomedical Engineering, National Cheng-Kung University, No. 1, Ta-Hseuh Road, Tainan 701, Taiwan, ROC;5. Center for Micro/Nano Technology Research, National Cheng-Kung University, Tainan 701, Taiwan, ROC;1. Programa de Pós-Graduação em Saúde Humana e Meio Ambiente, UFPE, 55608-680 Vitória de Santo Antão, PE, Brazil;2. Departamento de Bioquímica, UFPE, 50670-901 Recife, PE, Brazil;3. Departamento de Física, UFPE, 50670-901 Recife, PE, Brazil;1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100083, China;2. Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture, Beijing, 100083, China;1. Research Center for Bioengineering & Sensing Technology, School of Chemistry & Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China;2. Key Laboratory of Cosmetic, Beijing Technology & Business University, China National Light Industry, Beijing 100048, China;1. Department of Chemistry, University of Calgary, 2500 University Drive NW, Calgary, AB, Canada T2N 1N4;2. Defence Research and Development Canada, Suffield Research Centre, PO Box 4000, Station Main, Medicine Hat, AB, Canada T1A 8K6 |
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| Abstract: | Endotoxin can lead to irreversible shock and death, underlying the necessity for development of facile, rapid, sensitive and in situ methods to detect endotoxin. We used electrochemical impedance spectroscopy (EIS) to detect interaction of endotoxin with polymyxin B (PmB) immobilized on 4,4-dithiodibutyric acid coated gold electrodes. An equivalent circuit model with a constant phase element was used to interpret the obtained impedance spectra. The results indicated that the gold electrode after electrochemical polishing pretreatment in both 2-(N-Morpholino) ethanesulfonic acid (MES) and HClO4 solutions had a more effective surface state than that treated in H2SO4 solution. The changes in capacitance associated with a constant phase element on the PmB-modified electrodes were more sensitive, compared to those in the charge-transfer resistance, for detecting endotoxin over the 0.2–0.8 ng/mL concentrations. The activated and modified gold electrodes might be used to EIS detect endotoxin in real-time. |
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