Immobilization and electrochemical behavior of gold nanoparticles modified leukemia K562 cells and application in drug sensitivity test |
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| Institution: | 1. Jiangsu Institute of Cancer Prevention and Cure, Nanjing 210009, PR China;2. Key Laboratory of Analytical Chemistry for Life Science (Education Ministry of China), Department of Chemistry, Nanjing University, Nanjing 210093, China;1. Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan Province 475004, PR China;2. Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia;1. Department of Chemistry, University of Patras, 26500 Patras, Greece;2. Foundation of Research and Technology-Hellas, Institute of Chemical Engineering Sciences (FORTH/ICE-HT), Patras 26504, Greece;1. Institute for Frontier Materials, Deakin University, VIC 3220, Australia;2. CSIRO Materials Science and Engineering, Melbourne, VIC 3169, Australia;1. Anhui Provincial Key Laboratory of Photonic Devices and Materials, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China;2. Key Lab of Novel Thin Film Solar Cells, Chinese Academy of Sciences, Hefei 230031, China;3. School of Environmental Science and Optoelectronic Technology, University of Science and Technology of China, Hefei 230026, China |
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| Abstract: | A new strategy for immobilization of tumor cells on electrode surface and accelerating electron transfer between electrode and the immobilized cells was proposed to study the electrochemical behavior of cells and the effect of antitumor drug on cell viability. The leukemia K562 cells immobilized in a microporous cellulose membrane were firstly modified with colloidal gold nanoparticles to retain efficiently the activity of immobilized living tumor cells and promote electron transfer between electroactive centers of the cells and the electrode, exhibiting a well-defined anodic peak of guanine at +0.830 V at 50 mV s?1. The electrochemical response could be used to describe cell growth and evaluate the effectiveness of antitumor drug methotrexate on tumor cells. The proposed method offered potential advantages for drug sensitivity test with little usage of cells. It could be developed as a convenient means for the study of the tumor cells growth and the cytotoxicity of antitumor drugs. |
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