Quantum-dots based electrochemical immunoassay of interleukin-1α |
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| Institution: | 1. Department of Physics, Thanthai Periyar Government Institute of Technology, Vellore, India;2. Department of Chemistry, Government College of Technology, Coimbatore, India;1. Universidad Autonoma de San Luis Potosi, Coordinacion para la Innovacion y la Aplicacion de la Ciencia y la Tecnologia, San Luis Potosi, Mexico;2. Universidad Autonoma de San Luis Potosi, Facultad de Ciencias Quimicas, San Luis Potosi, Mexico;1. 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal;2. ICVS/3B’s – PT Government Associate Laboratory, Braga/Guimarães, Portugal;3. Biomedical Engineering, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands;4. Department of Veterinary Sciences, University of Trás-os-Montes e Alto Douro, Vila Real, Portugal;1. Instituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez, Ave. Del Charro #610 norte, Col. Partido Romero, C.P. 32320 Cd. Juárez, Chihuahua, México;2. Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Anillo envolvente del PRONAF y Estocolmo, C.P. 32320 Cd. Juárez, Chihuahua, México |
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| Abstract: | We describe a quantum-dot (QD, CdSe@ZnS) based electrochemical immunoassay to detect a protein biomarker, interleukin-1α (IL-1α). QD conjugated with anti-IL-1α antibody was used as a label in an immunorecognition event. After a complete sandwich immunoreaction among the primary IL-1α antibody (immobilized on the avidin-modified magnetic beads), IL-1α, and the QD-labeled secondary antibody, QD labels were attached to the magnetic-bead surface through the antibody-antigen immunocomplex. Electrochemical stripping analysis of the captured QDs was used to quantify the concentration of IL-1α after an acid-dissolution step. The streptavidin-modified magnetic beads and the magnetic separation platform were used to integrate a facile antibody immobilization (through a biotin/streptavidin interaction) with immunoreactions and the isolation of immunocomplexes from reaction solutions in the assay. The voltammetric response is highly linear over the range of 0.5–50 ng ml−1 IL-1α, and the limit of detection is estimated to be 0.3 ng ml−1 (18 pM). This QD-based electrochemical immunoassay shows great promise for rapid, simple, and cost-effective analysis of protein biomarkers. |
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