Immobilization and electrochemistry of cytochrome c on amino-functionalized mesoporous silica thin films |
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| Affiliation: | 1. School of Chemistry, Monash University, Clayton, Victoria 3800, Australia;2. School of Applied Sciences, RMIT University, GPO Box 2476V, VIC 3001, Melbourne, Australia;3. Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC 3052, Australia;4. School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, GPO Box 2434, QLD 4001, Brisbane, Australia;1. Department of Surgery, Section of Transplant Surgery, Suite 6-C, Wayne State University School of Medicine, 4201 St. Antoine BLVD, Detroit, MI 48201, United States;2. Department of Biomedical Engineering, Smart Sensor and Integrated Microsystems, 3100 Eng. Bldg, 5050 Anthony Wayne Drive, Detroit, MI 48202, United States;3. Department of Biomedical Engineering, Smart Sensor and Integrated Microsystems, 3100 Eng. Bldg, 5050 Anthony Wayne Drive, Detroit, MI 48202, United States |
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| Abstract: | The immobilization and electrochemistry of cytochrome c (cyt c) on amino-functionalized mesoporous silica thin films are described. The functionalized silica films with an Im3m cubic phase structure were deposited on conducting ITO substrate by co-condensation of tetraethoxysilane (TEOS) and 3-aminopropyltriethoxysilane (APTES) in the presence of Pluronic F127 under acidic conditions. The high specific surface area, large pore size and functional inner surface of mesoporous silica thin films result in a high cyt c loading, and the cyt c immobilization on this silicate framework is stable. After adsorption of cyt c, the ordered cubic structure of mesoporous silica and the redox activity of immobilized cyt c are retained as demonstrated by X-ray diffraction (XRD), Transmission electron microscope (TEM) and cyclic voltammetry. The redox behavior of the cyt c/silica film-modified ITO electrode is a surface-controlled quasi-reversible process for the experimental conditions used in this work and the electron transfer rate constant is calculated is 1.33 s−1. The ITO electrode modified by cyt c/silica film possesses a high stability; even cyt c retains its redox activity following immobilization for several months. Furthermore, the electrocatalytic activities of the modified ITO electrode to hydrogen peroxide and ascorbic acid have been studied. Since these behaviors are quite pronounced, the modified electrode can be used for detection of hydrogen peroxide and ascorbic acid. |
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