Microanalysis of nucleic acids using the limulus G test. |
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| Authors: | Takahisa Anada Kazuya Koumoto Masami Mizu Ryouji Karinaga Kazuo Sakurai Seiji Shinkai |
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| Affiliation: | Department of Chemical Process and Environments, The University of Kitakyushu, 1-1, Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0135, Japan. |
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| Abstract: | The limulus G test has been used as a quantitative analysis of (1-->3)-beta-D-glucans, including schizophyllan (SPG) and curdlan. The present work extended the limulus G test to detect polynucleotide/SPG complexes. The complex showed an extremely sensitive response to the test, compared with SPG itself. The minimum concentration of the complex to show the response is almost 10-times as small as that of SPG itself, indicating the possibility to detect (1-->3)-beta-D-glucans or/and polynucleotides on the pico gram/ml scale. |
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