荧光碳点的合成及对酿酒酵母的毒性研究

以葡萄糖为碳源采用溶剂热法合成了荧光碳点。紫外吸收光谱、荧光光谱以及透射电镜照片表明,所合成的荧光碳点发光性能优异,分散性好,且无团聚现象。荧光碳点原溶液出现浓度淬灭现象,稀释60倍情况下荧光最强。以酿酒酵母为模型生物,考察了不同生长时期(调整期、对数期早期、对数期中期)的酿酒酵母与不同浓度的荧光碳点共培养后的生长曲线。结果表明,即使荧光碳点浓度在27.75 mmol.L-1条件下也没有影响酵母菌的生长曲线,可认为基本没有细胞毒性。比较了相同荧光强度下的荧光碳点与CdTe量子点对酿酒酵母的细胞毒性,结果表明荧光碳点的毒性显著低于量子点的毒性。

Preparation of Fluorescent Carbon Dots and Its Cytotoxicity for Saccharomyce cerevisiae

Fluorescent carbon dots(CDs) were synthesized by a solvothermal method with glucose as the carbon source.UV absorption and fluorescence spectroscopy,and transmission electron microscopy(TEM) results showed that the synthesized CDs feature the excellent optical performance and high dispersion without agglomeration.The as-prepared CDs solution displayed a concentration quenching phenomenon and emitted the strongest fluorescence after being diluted 60 times.By using Saccharomyce cerevisiae yeast as a model organism,we investigated the dependence of yeast′s growth on the concentration of fluorescent CDs at various growth periods(adjustment,initial and middle logarithmic phases).It is shown that there is no influence on yeast′s growth curve even the concentration of fluorescent CDs as high as 27.75 mmol·L-1.The yeast cytotoxicity of fluorescent CDs and CdTe quantum dots were also compared under the same fluorescent intensity,indicating a much lower cytotoxicity fluorescent of CDs than that CdTe quantum dots.