Reversible photopadlocking on double-stranded DNA |
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| Authors: | Fujimoto Kenzo Matsuda Shigeo Yoshimura Yoshinaga Ami Takehiro Saito Isao |
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| Affiliation: | School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, 923-1292, Japan. kenzo@jaist.ac.jp |
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| Abstract: | We describe a highly efficient method for reversible photocircularization of oligonucleotide (ODN) on a double-stranded DNA template: 5-carboxyvinyl-2'-deoxyuridine-containing ODN was reversibly circularized around the target sequence of the double-stranded plasmid DNA resulting in formation of a catenated plasmid. |
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