Prilocaine-cyclodextrin-liposome: effect of pH variations on the encapsulation and topology of a ternary complex using 1H NMR |
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Authors: | Cabeça Luís Fernando Figueiredo Isis Martins de Paula Eneida Marsaioli Anita Jocelyne |
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Institution: | Institute of Chemistry, UNICAMP, PO Box 6154, 13083-970 Campinas, SP, Brazil. |
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Abstract: | A better comprehension of the prilocaine (PLC)-β-cyclodextrin (β-CD) complex liberation to membranes was provided by studying the architectural supramolecular arrangements of PLC, β-CD and egg phosphatidylcholine (EPC) liposomes, a membrane model. The topologies and possible interactions of mixtures of PLC, β-CD and EPC liposomes were investigated by nuclear magnetic resonances combining experimental (1)H-NMR (1D ROESY, STD and DOSY) at different pHs. The results indicate that in the mixture PLC/β-CD/EPC at pH 10 the PLC molecules are almost totally embedded into the liposomes and little interaction was observed between PLC and β-CD. However, at pH 5.5 not only was PLC imbedded in the EPC bilayer, but PLC was also interacting with β-CD. These results were rationalized as a spontaneous PLC release from β-CD to liposomes vesicles, whereas the PLC/EPC complex formation was higher at pH 10 than pH 5.5. |
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Keywords: | NMR 1H 1D ROESY DOSY STD prilocaine cyclodextrins liposome |
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