STATIC and TIME-RESOLVED FLUORESCENCE OF AN AMPHIPHILIC FLAVIN IN AEROSOL OT REVERSED MICELLES |
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Authors: | Antonie J W G Visser Kees Vos Jillert S Santema Jan Bouwstra Arie VAN Hoek |
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Institution: | Department of Biochemistry, Agricultural University, De Dreyen 11, 6703 BC Wageningen, The Netherlands |
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Abstract: | Spectral and time-resolved fluorescence studies have been carried out on N(3)-undecyllumi-flavin dispersed in reversed micelles composed of the surfactant sodium di(2-ethylhexyl) sulfosuccinate (Aerosol OT, AOT), various amounts of water and n -heptane as continuous phase. The fluorescence spectral properties (spectral distribution, quantum efficiencies and lifetimes) as function of the water to AOT molar ratio suggest that the flavin occupies a position within the surfactant boundary layer in close contact with water. The fluorescence anisotropy exhibits biexponential decay with a short (0.3 ns) and a longer (1.6–2.4 ns) correlation time. The contribution of the short component increases with the growth of the droplet providing evidence for enhanced flexibility of the flavin in the interfacial layer. |
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