Synthesis of 4-Benzidinyl Butyric Acid: A Key Intermediate for Antibodies Production Against Benzidin |
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| Authors: | Nathalie Fischer-Durand Samy Ben Rejeb François Le Goffic |
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| Institution: | Laboratoire de Bioorganique et Biotechnologies associé au CNRS , E.N.S.C.P. 11, rue P. et M. Curie, cedex 05, 75231, Paris, France |
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| Abstract: | a four-step synthesis of monoalkylated benzidin is reported for immunogen preparation against benzidin dye. Use of 10% H2SO4/ dioxan for N-Boc cleavage avoids the cyclisation of 4-benzidinyl butyric acid into the corresponding lactam observed with the classical TFA/ CH2Cl2 system. |
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