肺Ca2+含量与硅肺的关系

本文用离子选择电极测定了实验性诱发硅肺大鼠的肺、脑、肾、肝和血清中的[Ca~(2+)]、[K~+]和[Na~+],还以荧光探针Tb~(3+)探讨了Ca~(2+)的作用。在硅肺形成后,大鼠的肺、脑、肾、肝和血清等组织中的游离[Ca~(2+)]均发生变化。荧光探针表明:硅肺大鼠肺Ca~(2+)增加部分来自线粒体Ca~(2+)的释放硅肺。大鼠肺中游离Ca~(2+)和线粒体Ca~(2+)的变化可能与巨噬细胞的损伤有关。

CALCIUM CONTENT OF LUNG AND SILICOSIS

The variation of inorganic ions in silicosis has not been studied. As an extension of our previous studies, the concentrations of Ca²⁺, h and Na⁺ in lung, brain, kidney, liver and serum of rats with experimently silica-induced silicosis were measured by ion selective electrodes and the effect of Ca²⁺ was studied by fluorescent probe Tb³⁺. The following findings are obtained:1. The rats with experimental silicosis induced by intratracheal injection of SiO₂ crytalline dusts grew up much more slowly and their bron-chopulmonary lymph node gave rise to granulomas and weight of the lung increased.2. The concentration of free calcium ion in lung of silicotic rats was higher than that of the control group. The increase seemed to have reached its maximum within the first two months after injection of SiO₂ crystalline dusts into the right lung. From second to fifth month afterwards, the increased concentration of Ca²⁺ did not change noticeably with time. This is true for the infected right lung and diaphromatic lung. But for the left lung, the increase was noticed only at the fifth month.3. For the silicotic rats, concentration of Ca²⁺ ion in the front segment brain, liver and kidney were considerably increased but that of the serum was greatly decreased. However, at the fifth month these differences disappeared with the exception of the case of the brain wherein increase of Ca²⁺ concentration became more marked.4. By use of fluorescent probe Tb8+,it was found that the content of Ca²⁺ of mitochondria in the right lung and front segment brain of silicotic rats was higher than that of the control group. But the content of Ca²⁺ of mitochondria in the bronchopulmonary lymph node decreased with the time after the injection of SiO₂ crystalline dusts.5. Concentration of the free K⁺ ion and Na⁺ ion in lung, brain, liver and serum of silicotic rats had no marked difference as compared with those of the control group.All above findings had been initially explained.