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| 多指标综合研究返魂草提取物的提取工艺 | |
| 引用本文： | 牛天增,孟月娟,林华,张小平,孟祥颖,鲍永利,乌垠,于春雷,孙颖,李玉新.多指标综合研究返魂草提取物的提取工艺[J].东北师大学报(自然科学版),2009,41(4). |
| 作者姓名： | 牛天增 孟月娟 林华 张小平 孟祥颖 鲍永利 乌垠 于春雷 孙颖 李玉新 |
| 作者单位： | 1. 东北师范大学遗传与细胞研究所,吉林,长春,130024 2. 东北师范大学药物基因和蛋白筛选国家工程实验室,吉林,长春,130024 3. 东北师范大学药物基因和蛋白筛选国家工程实验室,吉林,长春,130024;东北师范大学国家教育部农业与医药基因工程研究中心,吉林,长春,130024 |
| 基金项目： | 长春市科技发展计划项目，吉林省科技发展计划项目，吉林省中医药局资助项目，吉林修正药业委托资助项目 |
| 摘 要： | 利用L_9(3~4)正交试验设计表,采用绿原酸、咖啡酸、异槲皮苷三种单体为含量测定指标,同时考虑总固体物得率,探讨了返魂草提取工艺中的几个影响因素,并对实验结果进行极差和方差分析.实验结果表明,提取次数为3次、90℃条件下、乙醇质量分数为60%、每次提取6 h为最佳提取工艺.该工艺操作简便、稳定可靠,为返魂草新制剂的研究提供了参考. |
| 关 键 词： | 返魂草 正交设计 绿原酸 咖啡酸 异槲皮苷 |
Multi-index synthetic study on extracting technology for the extract of Senecio cannabifolius Less. |
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| NIU Tian-zeng,MENG Yue-juan,LIN Hua,ZHANG Xiao-ping,MENG Xiang-ying,BAO Yong-li,WU Yin,YU Chun-lei,SUN Ying,LI Yu-xin.Multi-index synthetic study on extracting technology for the extract of Senecio cannabifolius Less.[J].Journal of Northeast Normal University (Natural Science Edition),2009,41(4). | |
| Authors: | NIU Tian-zeng MENG Yue-juan LIN Hua ZHANG Xiao-ping MENG Xiang-ying BAO Yong-li WU Yin YU Chun-lei SUN Ying LI Yu-xin |
| Institution: | NIU Tian-zeng1,MENG Yue-juan2,LIN Hua2,ZHANG Xiao-ping1,MENG Xiang-ying1,BAO Yong-li2,3,WU Yin2,YU Chun-lei2,SUN Ying2,LI Yu-xin1(1.Insititute of Genetic , Cytology,Northeast Normal University,Changchun 130024,China,2.National Engineering laboratory for Druggable Gene , Protein Screening,NorthEast Normal University1,3.Research Center of Agriculture , Medicine Gene Engineering of Ministry of Education,NorthEast Normal University 2,China) |
| Abstract: | A reasonable extraction process to the chemical constituents of Senecio cannabifolius Less.was designed.Method of L9(34) orthogonal design was employed with content of effective constituents chlorogenic acid,caffeic acid,isoquercitrin as assaying investigating indexes first time and regarding total solids yield simultaneously.The three compounds' content was determinated by RP-HPLC and approached several influencing factors of extracting technology of Senecio cannabifolius Less..Test result was detected wit... |
| Keywords: | Senecio cannabifolius Less orthogonal design chlorogenic acid caffeic acid isoquercitrin |
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