Synthesis of [18,19,21,22‐13C4]‐Labeled Tautomycin as an NMR Probe of Protein Phosphatase Inhibitor |
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| Authors: | Minoru Isobe Prof Dr Masakuni Kurono Dr Katsunori Tsuboi Dr Akira Takai Prof Dr |
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| Institution: | 1. Laboratory of Organic Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464‐8601, Japan, Fax: (+81)?52‐789‐4111;2. Asahikawa Medical College, Midorigaoka Higashi 2‐1‐1‐1, Asahikawa 078‐8510, Japan |
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| Abstract: | We have accomplished the synthesis of 13C‐labeled tautomycin at the C18, C19, C21, and C22 positions starting from 100 % 13C]triethylphosphonoacetate for the purpose of elucidating the dynamics and conformation of the C17–C26 moiety. NMR spectroscopy of 13C‐labeled tautomycin revealed strong binding with protein phosphatase type 1 and new features in the 13C NMR spectrum, such as the very small three‐bond coupling constants (2J). |
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| Keywords: | enzyme inhibitors isotope labeling NMR spectroscopy protein phosphatase tautomycin |
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