Hemolytic activity of polyoxyethylene cholesteryl ethers |
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Authors: | K Miyajima T Baba M Nakagaki |
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Institution: | (1) Present address: Faculty of Pharmaceutical Sciences, Kyoto University, Japan |
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Abstract: | Hemolytic activity of nonionic surfactants, polyoxyethylene cholesteryl ethers, C27H45O(CH2CH2O)
n
H (Chol-E
n
,n=, 25, 30, 50) and polyoxyethylene dihydrocholeseryl ethers, C27H47O(CH2CH2O)
n
H (DHChol-E
n
,n=15, 30 50) were measured, changing the concentration of surfactant and erythrocyte at 37 °C. Maximum hemolytic activity was observed in these cholesteryl derivatives with 25–30 oxyethylene units. The time course of hemolysis was also measured as a function of the concentrations of surfactant and erythrocyte. Hemolysis started after a certain induction period, , and then apparently proceeded as a first-order reaction with respect to the erythrocyte concentration. The surfactant inducing 50% hemolysis at low concentration had a small value and large rate constant. The maximum amount of adsorption without inducing hemolysis,a
0, decreased with increasing polyoxyethylene chain length. Chol-E25 has the maximum activity for the solubilization of egg yolk lecithin at 37 °C. Based on these results, the mechanism of hemolysis by these surfactants was quantitatively discussed. |
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Keywords: | Polyoxyethylene cholesterylethers hemolysis bovineerythrocyte criticalmicelle concentration |
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