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Enhancing resolution of free‐flow zone electrophoresis via a simple sheath‐flow sample injection
Authors:Ying Yang  Fan‐Zhi Kong  Ji Liu  Jun‐Min Li  Xiao‐Ping Liu  Guo‐Qing Li  Ju‐Fang Wang  Hua Xiao  Liu‐Yin Fan  Shan Li
Institution:1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, P. R. China;2. Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China
Abstract:In this work, a simple and novel sheath‐flow sample injection method (SFSIM) is introduced to reduce the band broadening of free‐flow zone electrophoresis separation in newly developed self‐balance free‐flow electrophoresis instrument. A needle injector was placed in the center of the separation inlet, into which the BGE and sample solution were pumped simultaneously. BGE formed sheath flow outside the sample stream, resulting in less band broadening related to hydrodynamics and electrodynamics. Hemoglobin and C‐phycocyanin were successfully separated by the proposed method in contrast to the poor separation of free‐flow electrophoresis with the traditional injection method without sheath flow. About 3.75 times resolution enhancement could be achieved by sheath‐flow sample injection method.
Keywords:Band broadening  Free‐flow zone electrophoresis  Sample injection  Sheath flow
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