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Antibacterial and Antifungal Studies of Biosynthesized Silver Nanoparticles against Plant Parasitic Nematode Meloidogyne incognita,Plant Pathogens Ralstonia solanacearum and Fusarium oxysporum
Authors:Masudulla Khan  Azhar U. Khan  Nina Bogdanchikova  Diana Garibo
Affiliation:1.School of Life and Basic Sciences, SIILAS, Jaipur National University, Jaipur 302017, India;2.Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, 22800 Ensenada, Baja California, Mexico;3.CONACYT Research Fellow at Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, 22800 Ensenada, Baja California, Mexico
Abstract:The possibility of using silver nanoparticles (AgNPs) to enhance the plants growth, crop production, and control of plant diseases is currently being researched. One of the most effective approaches for the production of AgNPs is green synthesis. Herein, we report a green and phytogenic synthesis of AgNPs by using aqueous extract of strawberry waste (solid waste after fruit juice extraction) as a novel bioresource, which is a non-hazardous and inexpensive that can act as a reducing, capping, and stabilizing agent. Successful biosynthesis of AgNPs was monitored by UV-visible spectroscopy showing a surface plasmon resonance (SPR) peak at ~415 nm. The X-ray diffraction studies confirm the face-centered cubic crystalline AgNPs. The scanning electron microscopy (SEM) and transmission electron microscopy (TEM) techniques confirm the rectangular shape with an average size of ~55 nm. The antibacterial and antifungal efficacy and inhibitory impact of the biosynthesized AgNPs were tested against nematode, Meloidogyne incognita, plant pathogenic bacterium, Ralstonia solanacearum and fungus, Fusarium oxysporum. These results confirm that biosynthesized AgNPs can significantly control these plant pathogens.
Keywords:silver nanoparticles   antibacterial studies   antifungal studies   strawberry waste   Fusarium oxysporum   Ralstonia solanacearum
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