Synthesis,Quantification, and Characterization of Fatty Acid Amides from In Vitro and In Vivo Sources |
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| Authors: | Ruidong Ni Suzeeta Bhandari Perry R. Mitchell Jr. Gabriela Suarez Neel B. Patel Kara Lamb Kirpal S. Bisht David J. Merkler |
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| Affiliation: | Department of Chemistry, University of South Florida, 4202 E. Fowler Ave., Tampa, FL 33620, USA; (R.N.); (S.B.); (P.R.M.J.); (G.S.); (N.B.P.); (K.L.) |
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| Abstract: | Fatty acid amides are a diverse family of underappreciated, biologically occurring lipids. Herein, the methods for the chemical synthesis and subsequent characterization of specific members of the fatty acid amide family are described. The synthetically prepared fatty acid amides and those obtained commercially are used as standards for the characterization and quantification of the fatty acid amides produced by biological systems, a fatty acid amidome. The fatty acid amidomes from mouse N18TG2 cells, sheep choroid plexus cells, Drosophila melanogaster, Bombyx mori, Apis mellifera, and Tribolium castaneum are presented. |
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| Keywords: | fatty acid amide liquid chromatography/quadrupole time-of-flight mass spectrometry anandamide N18TG2 choroid plexus Drosophila Bombyx Apis Tribolium |
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