| 高效液相色谱法测定水产品中7种兽药残留量 |
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| 引用本文： | 孟勇,张美琴,王静,谭秀慧,吴光红.高效液相色谱法测定水产品中7种兽药残留量[J].理化检验(化学分册),2012(5):543-546. |
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| 作者姓名： | 孟勇 张美琴 王静 谭秀慧 吴光红 |
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| 作者单位： | 农业部渔业产品质量监督检验测试中心;江苏省水产质量检测中心 |
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| 基金项目： | 江苏省科技基础设施建设计划(BM2008158);江苏省水产三项工程(PJ2020-52) |
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| 摘 要： | 提出了高效液相色谱法同时测定水产品中呋喃唑酮、噁喹酸、萘啶酸及4种磺胺类药物等7种兽药残留量的方法。样品经乙腈提取,正己烷脱脂,用中性氧化铝柱和C18固相萃取柱进行分离及净化。以Symmetry C18色谱柱为分离柱,以不同体积比混合的乙腈与0.08mol.L-1乙酸溶液为流动相进行梯度洗脱,紫外检测器于波长265nm处检测。7种兽药的质量浓度与其峰面积均在0.05～1.0mg.L-1范围内呈线性关系,检出限(3S/N)为0.01～0.04mg.kg-1。方法的回收率在68.2%～97.3%之间,相对标准偏差(n=6)在1.6%～14%之间。
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| 关 键 词： | 呋喃唑酮 噁喹酸 萘啶酸 磺胺类药物 高效液相色谱法 |
HPLC Determination of Residual Amounts of 7 Veterinary Drugs in Aquatic Products |
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| MENG Yong,ZHANG Mei-qing,WANG Jing,TAN Xiu-hui,WU Guang-hong.HPLC Determination of Residual Amounts of 7 Veterinary Drugs in Aquatic Products[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2012(5):543-546. |
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| Authors: | MENG Yong ZHANG Mei-qing WANG Jing TAN Xiu-hui WU Guang-hong |
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| Institution: | 1,2(1.Nanjing Quality Supervision and Testing Center for Fishery Products,Ministry of Agriculture,Nanjing 210017,China; 2.Jiangsu Provincial Inspection Center for Quality of Aquatic Products,Nanjing 210017,China) |
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| Abstract: | A method of HPLC for the determination of residual amounts of 7 veterinary drugs,including furazolidone,oxolinic acid,nalidixic acid and 4 sulfonamides in aquatic products was proposed.The sample was extracted with acetonitrile and defatted with n-hexane.The residue obtained was taken up with CH3CN-H2O(95+5) and purified by passing through neutral alumina column and C18 column.The eluate was separated on Symmetry C18 column by gradient elution with mixtures of acetonitrile and 0.08 mol·L-1 acetic acid solution in different ratios,and determined with UV detector at the wavelength of 265 nm.Linear relationships between values of peak area ad mass concentration of the 7 veterinary drugs were obtained in the same range of 0.05-10.0 mg·L-1,with detection limits(3S/N) in the range of 0.01-0.04 mg·kg-1.Values of recovery and RSD′s(n=6) of the method found were in the ranges of 68.2%-97.3% and 1.6%-14% respectively. |
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| Keywords: | Furazolidone Oxolinic acid Nalidixic acid Sulfonamides HPLC |
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