CSF from Parkinson disease Patients Differentially Affects Cultured Microglia and Astrocytes |
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Authors: | Mya C Schiess Jennifer L Barnes Timothy M Ellmore Brian J Poindexter Kha Dinh and Roger J Bick |
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Institution: | 1.Department of Neurology,University of Texas Medical School at Houston,Houston,USA;2.Department of Neurosurgery,University of Texas Medical School at Houston,Houston,USA;3.Department of Pathology and Laboratory Medicine,University of Texas Medical School at Houston,Houston,USA |
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Abstract: | Background Excessive and abnormal accumulation of alpha-synuclein (α-synuclein) is a factor contributing to pathogenic cell death in
Parkinson's disease. The purpose of this study, based on earlier observations of Parkinson's disease cerebrospinal fluid (PD-CSF)
initiated cell death, was to determine the effects of CSF from PD patients on the functionally different microglia and astrocyte
glial cell lines. Microglia cells from human glioblastoma and astrocytes from fetal brain tissue were cultured, grown to confluence,
treated with fixed concentrations of PD-CSF, non-PD disease control CSF, or control no-CSF medium, then photographed and fluorescently
probed for α-synuclein content by deconvolution fluorescence microscopy. Outcome measures included manually counted cell growth
patterns from day 1-8; α-synuclein density and distribution by antibody tagged 3D model stacked deconvoluted fluorescent imaging. |
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