Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography-tandem mass spectrometry |
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Authors: | Ding Cun-Gang Zhou Zhen Ge Qing-Hua Zhi Xiao-Jin Ma Li-Li |
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Institution: | Shanghai Institute of Pharmaceutical Industry, 1111 Zhongshan North One Road, Shanghai 200437, People's Republic of China. |
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Abstract: | A method has been developed for the simultaneous quantification of metformin (I) and glipizide (II) in human plasma. It is based on high-performance liquid chromatography with electrospray ionization tandem mass (LC-ESI-MS/MS) spectrometric detection in positive ionization mode. Phenformin (III) and gliclazide (IV) were used as internal standards for I and II, respectively. The MS/MS detection was performed in multiple reaction monitoring (MRM) mode. The precursor-product ion combinations of m/z 130 --> 71, 446 --> 321, 206 --> 60 and 324 --> 127 were used to quantify I, II, III and IV, respectively. This method was validated in the concentration ranges of 0.02-4 microg/mL for I and 0.004-0.8 microg/mL for II. It was utilized to support a clinical pharmacokinetic study after single dose oral administration of a combination of I and II. |
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Keywords: | metformin glipizide LC‐MS/MS ESI human plasma determination |
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