Preparation of stimuli-responsive protein nanogel by quantum-ray irradiation

To prepare the functional nanoparticle with biological affinity, we tried to control the particle diameter and volume phase transition point of protein nanogel by quantum-ray irradiation. We succeeded in controlling the particle diameter of gelatin nanogel in the range of 20–70 nm by gamma-ray irradiation. It was also found that the prepared gelatin nanogel reversibly swelled and shrunk by pH and temperature change. Volume phase transition point and swelling ratio were found to change, depending on the absorbed dose and gelatin concentration.